LOCUS       V009982                 4969 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009982
VERSION     V009982
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Han T, Chen Q, Liu H.
  TITLE     Engineered photoactivatable genetic switches based on the bacterium
            phage T7 RNA polymerase
  JOURNAL   ACS Synth Biol (2016) In press
   PUBMED   27794600
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Han T, Chen Q, Liu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2016) School of Life Sciences, University of
            Science and Technology of China, University of Science and
            Technology of China, No.96, JinZhai Road Baohe District,Hefei,Anhui,
            230026,P.R.China, Hefei, Anhui 230026, China
REFERENCE   3  (bases 1 to 4969)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4969)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI v. Vector NTI Advance (TM) 11.0
            Assembly Name         :: full-length T7 RNAP
            Coverage              :: 2323bp
            Sequencing Technology :: 454
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
            Biol (2016) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-OCT-2016) School of Life Sciences, University of Science and
            Technology of China, University of Science and Technology of China,
            No.96, JinZhai Road Baohe District,Hefei,Anhui, 230026,P.R.China,
            Hefei, Anhui 230026, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..116)
                     /label="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     regulatory      125..162
                     /regulatory_class="promoter"
     regulatory      179..190
                     /regulatory_class="ribosome_binding_site"
     RBS             179..190
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             197..2845
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     regulatory      2873..2906
                     /regulatory_class="terminator"
     misc_feature    2906..2926
                     /label="BioBrick suffix"
                     /note="universal suffix for all parts"
     terminator      2927..2984
                     /label="his operon terminator"
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(3179..3767)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4003..4815)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
       61 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctggc
      121 tagcggcacg taagaggttc caactttcac cataatgaaa caccatgggg tactagagaa
      181 agaggagaaa agatctatga acacgattaa catcgctaag aacgacttct ctgacatcga
      241 actggctgct atcccgttca acactctggc tgaccattac ggtgagcgtt tagctcgcga
      301 acagttggcc cttgagcatg agtcttacga gatgggtgaa gcacgcttcc gcaagatgtt
      361 tgagcgtcaa cttaaagctg gtgaggttgc ggataacgct gccgccaagc ctctcatcac
      421 taccctactc cctaagatga ttgcacgcat caacgactgg tttgaggaag tgaaagctaa
      481 gcgcggcaag cgcccgacag ccttccagtt cctgcaagaa atcaagccgg aagccgtagc
      541 gtacatcacc attaagacca ctctggcttg cctaaccagt gctgacaata caaccgttca
      601 ggctgtagca agcgcaatcg gtcgggccat tgaggacgag gctcgcttcg gtcgtatccg
      661 tgaccttgaa gctaagcact tcaagaaaaa cgttgaggaa caactcaaca agcgcgtagg
      721 gcacgtctac aagaaagcat ttatgcaagt tgtcgaggct gacatgctct ctaagggtct
      781 actcggtggc gaggcgtggt cttcgtggca taaggaagac tctattcatg taggagtacg
      841 ctgcatcgag atgctcattg agtcaaccgg aatggttagc ttacaccgcc aaaatgctgg
      901 cgtagtaggt caagactctg agactatcga actcgcacct gaatacgctg aggctatcgc
      961 aacccgtgca ggtgcgctgg ctggcatctc tccgatgttc caaccttgcg tagttcctcc
     1021 taagccgtgg actggcatta ctggtggtgg ctattgggct aacggtcgtc gtcctctggc
     1081 gctggtgcgt actcacagta agaaagcact gatgcgctac gaagacgttt acatgcctga
     1141 ggtgtacaaa gcgattaaca ttgcgcaaaa caccgcatgg aaaatcaaca agaaagtcct
     1201 agcggtcgcc aacgtaatca ccaagtggaa gcattgtccg gtcgaggaca tccctgcgat
     1261 tgagcgtgaa gaactcccga tgaaaccgga agacatcgac atgaatcctg aggctctcac
     1321 cgcgtggaaa cgtgctgccg ctgctgtgta ccgcaaggac aaggctcgca agtctcgccg
     1381 tatcagcctt gagttcatgc ttgagcaagc caataagttt gctaaccata aggccatctg
     1441 gttcccttac aacatggact ggcgcggtcg tgtttacgct gtgtcaatgt tcaacccgca
     1501 aggtaacgat atgaccaaag gactgcttac gctggcgaaa ggtaaaccaa tcggtaagga
     1561 aggttactac tggctgaaaa tccacggtgc aaactgtgcg ggtgtcgata aggttccgtt
     1621 ccctgagcgc atcaagttca ttgaggaaaa ccacgagaac atcatggctt gcgctaagtc
     1681 tccactggag aacacttggt gggctgagca agattctccg ttctgcttcc ttgcgttctg
     1741 ctttgagtac gctggggtac agcaccacgg cctgagctat aactgctccc ttccgctggc
     1801 gtttgacggg tcttgctctg gcatccagca cttctccgcg atgctccgag atgaggtagg
     1861 tggtcgcgcg gttaacttgc ttcctagtga aaccgttcag gacatctacg ggattgttgc
     1921 taagaaagtc aacgagattc tacaagcaga cgcaatcaat gggaccgata acgaagtagt
     1981 taccgtgacc gatgagaaca ctggtgaaat ctctgagaaa gtcaagctgg gcactaaggc
     2041 actggctggt caatggctgg cttacggtgt tactcgcagt gtgactaagc gttcagtcat
     2101 gacgctggct tacgggtcca aagagttcgg cttccgtcaa caagtgctgg aagataccat
     2161 tcagccagct attgattccg gcaagggtct gatgttcact cagccgaatc aggctgctgg
     2221 atacatggct aagctgattt gggaatctgt gagcgtgacg gtggtagctg cggttgaagc
     2281 aatgaactgg cttaagtctg ctgctaagct gctggctgct gaggtcaaag ataagaagac
     2341 tggagagatt cttcgcaagc gttgcgctgt gcattgggta actcctgatg gtttccctgt
     2401 gtggcaggaa tacaagaagc ctattcagac gcgcttgaac ctgatgttcc tcggtcagtt
     2461 ccgcttacag cctaccatta acaccaacaa agatagcgag attgatgcac acaaacagga
     2521 gtctggtatc gctcctaact ttgtacacag ccaagacggt agccaccttc gtaagactgt
     2581 agtgtgggca cacgagaagt acggaatcga atcttttgca ctgattcacg actccttcgg
     2641 taccattccg gctgacgctg cgaacctgtt caaagcagtg cgcgaaacta tggttgacac
     2701 atatgagtct tgtgatgtac tggctgattt ctacgaccag ttcgctgacc agttgcacga
     2761 gtctcaattg gacaaaatgc cagcacttcc ggctaaaggt aacttgaacc tccgtgacat
     2821 cttagagtcg gacttcgcgt tcgcgtaagg tacccatatg ggatccccgc ggcgccgaaa
     2881 accccgcttc ggcggggttt tgccgcacta gtagcggccg ctgcagtccg gcaaaaaagg
     2941 gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc
     3001 aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     3061 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     3121 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     3181 tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     3241 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     3301 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     3361 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     3421 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3481 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3541 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3601 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3661 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3721 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3781 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3841 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3901 caatctaaag tatatatgag taaacttggt ctgacagctc gagtcccgtc aagtcagcgt
     3961 aatgctctgc cagtgttaca accaattaac caattctgat tagaaaaact catcgagcat
     4021 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
     4081 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
     4141 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     4201 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     4261 aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     4321 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
     4381 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     4441 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     4501 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     4561 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     4621 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     4681 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     4741 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg
     4801 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     4861 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     4921 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagctcgag
//
